› 6j49

X-ray diffraction
1.6Å resolution

Grafting VLADV sequence into OspAsm1

Released:
DOI: 10.2210/pdb6j49/pdb
PDB entry 6j49 coloured by chain, front view.
PDB entry 6j49 coloured by chain, side view.
PDB entry 6j49 coloured by chain, top view.
Source organism: Borreliella burgdorferi B31
Primary publication:
Grafting a short chameleon sequence from αB crystallin into a β-sheet scaffold protein.
Hori Y, Fujiwara H, Fujiwara W, Makabe K
Proteins 87 416-424 (2019)
PMID: 30684364

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer surface protein A Chain: O
Molecule details ›
Chain: O
Length: 251 amino acids
Theoretical weight: 26.36 KDa
Source organism: Borreliella burgdorferi B31
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CL66 (Residues: 27-273; Coverage: 96%)
Gene names: BB_A15, ospA
Sequence domains: Borrelia lipoprotein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 33.229Å b: 54.485Å c: 66.489Å
α: 90° β: 100.02° γ: 90°
R-values:
R R work R free
0.183 0.181 0.218
Expression system: Escherichia coli

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