› 6j58

X-ray diffraction
1.52Å resolution

Crystal structure of human HINT1 complexing with AP4A

Released:
DOI: 10.2210/pdb6j58/pdb
PDB entry 6j58 coloured by chain, front view.
PDB entry 6j58 coloured by chain, side view.
PDB entry 6j58 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Second messenger Ap4A polymerizes target protein HINT1 to transduce signals in FcεRI-activated mast cells.
Yu J, Liu Z, Liang Y, Luo F, Zhang J, Tian C, Motzik A, Zheng M, Kang J, Zhong G, Liu C, Fang P, Guo M, Razin E, Wang J
OpenAccess logo Nat Commun 10 4664 (2019)
PMID: 31604935

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine 5'-monophosphoramidase HINT1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49773 (Residues: 1-126; Coverage: 100%)
Gene names: HINT, HINT1, PKCI1, PRKCNH1
Sequence domains: HIT domain

Ligands and Environments

1 bound ligand:
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 78.721Å b: 46.422Å c: 64.251Å
α: 90° β: 94.9° γ: 90°
R-values:
R R work R free
0.157 0.157 0.17
Expression system: Escherichia coli

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Citations

4 review citations

Re-evaluation of Diadenosine Tetraphosphate (Ap4A) From a Stress Metabolite to Bona Fide Secondary Messenger.
Ferguson et al. (2020)
3 more