› 6j7e

X-ray diffraction
2.4Å resolution

Crystal Structure of Central domain of FleQ in complex with ATPgS and Mg

Released:
DOI: 10.2210/pdb6j7e/pdb
PDB entry 6j7e coloured by chain, front view.
PDB entry 6j7e coloured by chain, side view.
PDB entry 6j7e coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Sensor I Regulated ATPase Activity of FleQ Is Essential for Motility to Biofilm Transition in Pseudomonas aeruginosa.
Banerjee P, Jain D
ACS Chem Biol 14 1515-1527 (2019)
PMID: 31268665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator FleQ Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.6 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XCV0 (Residues: 142-399; Coverage: 53%)
Gene names: PA1097, fleQ
Sequence domains: Sigma-54 interaction domain

Ligands and Environments

3 bound ligands:
Ligand AGS 1 x AGS
Ligand MG 1 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P65
Unit cell:
a: 104.549Å b: 104.549Å c: 42.762Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.25
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Highly regulated, diversifying NTP-dependent biological conflict systems with implications for the emergence of multicellularity.
Kaur et al. (2020)
5 more

2 mentions without citation

The antiactivator FleN uses an allosteric mechanism to regulate σ54-dependent expression of flagellar genes in Pseudomonas aeruginosa.
Chanchal et al. (2021)
1 more