› 6jbx

X-ray diffraction
2.2Å resolution

Crystal structure of Streptococcus pneumoniae FabT in complex with DNA

Released:
DOI: 10.2210/pdb6jbx/pdb
PDB entry 6jbx coloured by chain, front view.
PDB entry 6jbx coloured by chain, side view.
PDB entry 6jbx coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Structural insights into repression of the Pneumococcal fatty acid synthesis pathway by repressor FabT and co-repressor acyl-ACP.
Zuo G, Chen ZP, Jiang YL, Zhu Z, Ding C, Zhang Z, Chen Y, Zhou CZ, Li Q
FEBS Lett 593 2730-2741 (2019)
PMID: 31291684

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120062 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH marR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 17.78 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DR18 (Residues: 1-144; Coverage: 100%)
Gene name: spr0376
Sequence domains: MarR family
DNA (5'-D(*AP*AP*TP*AP*GP*TP*TP*TP*GP*AP*CP*TP*GP*TP*CP*AP*AP*AP*TP*TP*AP*TP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 7.09 KDa
Source organism: Streptococcus pneumoniae
Expression system: Not provided
DNA (5'-D(*CP*AP*TP*AP*AP*TP*TP*TP*GP*AP*CP*AP*GP*TP*CP*AP*AP*AP*CP*TP*AP*TP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 7.02 KDa
Source organism: Streptococcus pneumoniae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TRD 2 x TRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 73.022Å b: 60.868Å c: 74.086Å
α: 90° β: 105.4° γ: 90°
R-values:
R R work R free
0.189 0.188 0.221
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

FabT, a Bacterial Transcriptional Repressor That Limits Futile Fatty Acid Biosynthesis.
Lambert et al. (2022)
7 other articles

2 mentions without citation

TrmB Family Transcription Factor as a Thiol-Based Regulator of Oxidative Stress Response.
Mondragon et al. (2022)
1 more