› 6jd1

Electron Microscopy
3.38Å resolution

Cryo-EM Structure of Sulfolobus solfataricus ketol-acid reductoisomerase (Sso-KARI) in complex with Mg2+, NADH, and CPD at pH7.5

Released:
DOI: 10.2210/pdb6jd1/pdb
PDB entry 6jd1 coloured by chain, front view.
PDB entry 6jd1 coloured by chain, side view.
PDB entry 6jd1 coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Use of Cryo-EM To Uncover Structural Bases of pH Effect and Cofactor Bispecificity of Ketol-Acid Reductoisomerase.
Chen CY, Chang YC, Lin BL, Lin KF, Huang CH, Hsieh DL, Ko TP, Tsai MD
J Am Chem Soc 141 6136-6140 (2019)
PMID: 30921515
Related structures: EMD-9801

Function and Biology Details

Reaction catalysed: 
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189048 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative ketol-acid reductoisomerase 2 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 333 amino acids
Theoretical weight: 37.23 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97YJ9 (Residues: 1-333; Coverage: 100%)
Gene names: SSO1322, ilvC-2, ilvC2
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAI 12 x NAI
2 bound ligands:
Ligand MG 36 x MG
Ligand 9TY 12 x 9TY
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.38Å
Relevant EMDB volumes: EMD-9801
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

2.7 Å cryo-EM structure of vitrified M. musculus H-chain apoferritin from a compact 200 keV cryo-microscope.
Hamdi et al. (2020)
2 more