› 6jda

X-ray diffraction
2.9Å resolution

Crystal structure of N-acetyl mannosmaine kinase in complex with N-acetylmannosamine in Pasteurella multocida

Released:
DOI: 10.2210/pdb6jda/pdb
PDB entry 6jda coloured by chain, front view.
PDB entry 6jda coloured by chain, side view.
PDB entry 6jda coloured by chain, top view.
Source organism: Pasteurella multocida
Primary publication:
Structure and Function of N‐Acetylmannosamine Kinases from Pathogenic Bacteria.
Thanuja G, Ramaswamy S
Acs Omega 5 30923-30926 (2020)

Function and Biology Details

Reaction catalysed: 
ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylmannosamine kinase Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 30.69 KDa
Source organism: Pasteurella multocida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CKB3 (Residues: 1-291; Coverage: 98%)
Gene names: PM1712, nanK
Sequence domains: ROK family

Ligands and Environments

3 bound ligands:
Ligand BM3 1 x BM3
Ligand ZN 1 x ZN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 103.816Å b: 173.732Å c: 48.06Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.247
Expression system: Escherichia coli BL21(DE3)

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