› 6jdg

X-ray diffraction
2.39Å resolution

Complexed crystal structure of PaSSB with ssDNA dT20 at 2.39 angstrom resolution

Released:
DOI: 10.2210/pdb6jdg/pdb
PDB entry 6jdg coloured by chain, front view.
PDB entry 6jdg coloured by chain, side view.
PDB entry 6jdg coloured by chain, top view.
Source organisms:
Primary publication:
Complexed crystal structure of SSB reveals a novel single-stranded DNA binding mode (SSB)3:1: Phe60 is not crucial for defining binding paths.
Huang YH, Lin ES, Huang CY
Biochem Biophys Res Commun 520 353-358 (2019)
PMID: 31604524

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120067 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Single-stranded DNA-binding protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 121 amino acids
Theoretical weight: 13.77 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P40947 (Residues: 1-115; Coverage: 70%)
Gene names: PA4232, ssb
Sequence domains: Single-strand binding protein family
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chains: E, F, G
Molecule details ›
Chains: E, F, G
Length: 20 nucleotides
Theoretical weight: 6.04 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P31
Unit cell:
a: 60.042Å b: 60.042Å c: 129.642Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.213 0.264
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

7 citation in other articles

A Complexed Crystal Structure of a Single-Stranded DNA-Binding Protein with Quercetin and the Structural Basis of Flavonol Inhibition Specificity.
Lin et al. (2022)
6 more

2 mentions without citation

Complexed crystal structure of SSB reveals a novel single-stranded DNA binding mode (SSB)3:1: Phe60 is not crucial for defining binding paths.
Huang et al. (2019)
1 more