› 6jdi

X-ray diffraction
1.95Å resolution

Central domain of FleQ H287N mutant in complex with ATPgS and Mg

Released:
DOI: 10.2210/pdb6jdi/pdb
PDB entry 6jdi coloured by chain, front view.
PDB entry 6jdi coloured by chain, side view.
PDB entry 6jdi coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Sensor I Regulated ATPase Activity of FleQ Is Essential for Motility to Biofilm Transition in Pseudomonas aeruginosa.
Banerjee P, Jain D
ACS Chem Biol 14 1515-1527 (2019)
PMID: 31268665

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator FleQ Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.58 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XCV0 (Residues: 142-399; Coverage: 53%)
Gene names: PA1097, fleQ
Sequence domains: Sigma-54 interaction domain

Ligands and Environments

2 bound ligands:
Ligand AGS 1 x AGS
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P65
Unit cell:
a: 105.065Å b: 105.065Å c: 42.642Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.185 0.254
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Highly regulated, diversifying NTP-dependent biological conflict systems with implications for the emergence of multicellularity.
Kaur et al. (2020)
7 more