› 6jdx

X-ray diffraction
2.28Å resolution

Crystal structure of AcrIIC2 dimer in complex with partial Nme1Cas9 preprocessed with protease alpha-Chymotrypsin

Released:
DOI: 10.2210/pdb6jdx/pdb
Model geometry
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PDB entry 6jdx coloured by chain, front view.
PDB entry 6jdx coloured by chain, side view.
PDB entry 6jdx coloured by chain, top view.
Source organism: Neisseria meningitidis 8013
Primary publication:
Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2.
Thavalingam A, Cheng Z, Garcia B, Huang X, Shah M, Sun W, Wang M, Harrington L, Hwang S, Hidalgo-Reyes Y, Sontheimer EJ, Doudna J, Davidson AR, Moraes TF, Wang Y, Maxwell KL
OpenAccess logo Nat Commun 10 2806 (2019)
PMID: 31243272

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111888 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AcrIIC2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 14.2 KDa
Source organism: Neisseria meningitidis 8013
Expression system: Escherichia coli BL21(DE3)
CRISPR-associated endonuclease Cas9 Chain: C
Molecule details ›
Chain: C
Length: 78 amino acids
Theoretical weight: 8.79 KDa
Source organism: Neisseria meningitidis 8013
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C9X1G5 (Residues: 1-77; Coverage: 7%)
Gene names: NMV_1993, cas9

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 55.374Å b: 77.236Å c: 107.601Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.222
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies.
Marino et al. (2020)
11 more

1 mention without citation

Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2.
Thavalingam et al. (2019)

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