› 6jfj

X-ray diffraction
1.93Å resolution

Crystal structure of Pullulanase from Paenibacillus barengoltzii complex with maltohexaose and alpha-cyclodextrin

Released:
DOI: 10.2210/pdb6jfj/pdb
PDB entry 6jfj coloured by chain, front view.
PDB entry 6jfj coloured by chain, side view.
PDB entry 6jfj coloured by chain, top view.
Source organism: Paenibacillus barengoltzii
Primary publication:
Structural basis of carbohydrate binding in domain C of a type I pullulanase from Paenibacillus barengoltzii.
Huang P, Wu S, Yang S, Yan Q, Jiang Z
Acta Crystallogr D Struct Biol 76 447-457 (2020)
PMID: 32355041

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101078 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glycosyl hydrolase family 13 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 675 amino acids
Theoretical weight: 75.18 KDa
Source organism: Paenibacillus barengoltzii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0C5GWS2 (Residues: 1-675; Coverage: 100%)
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
8 bound ligands:
Ligand GOL 4 x GOL
Ligand PEG 10 x PEG
Ligand PGE 2 x PGE
Ligand 1PE 1 x 1PE
Ligand TAM 2 x TAM
Ligand IMD 4 x IMD
Ligand CA 2 x CA
Ligand CL 8 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Unit cell:
a: 49.911Å b: 98.179Å c: 140.187Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.146 0.189
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Insulin Complexation with Cyclodextrins-A Molecular Modeling Approach.
Bucur et al. (2022)