› 6jh1

X-ray diffraction
3Å resolution

Crystal structure of bISG15/NS1B complex

Released:
DOI: 10.2210/pdb6jh1/pdb
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PDB entry 6jh1 coloured by chain, front view.
PDB entry 6jh1 coloured by chain, side view.
PDB entry 6jh1 coloured by chain, top view.
Source organisms:
Entry authors: Jiang YN, Wang XW

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126426 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Non-structural protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.88 KDa
Source organism: Influenza B virus (B/Lee/1940)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03502 (Residues: 1-103; Coverage: 37%)
Gene name: NS
Sequence domains: Influenza B non-structural protein (NS1)
Ubiquitin-like protein ISG15 Chains: C, D
Molecule details ›
Chains: C, D
Length: 154 amino acids
Theoretical weight: 17.31 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O02741 (Residues: 1-154; Coverage: 100%)
Gene names: G1P2, ISG15, ISG17, UCRP
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P212121
Unit cell:
a: 59.14Å b: 80.05Å c: 109.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.274
Expression system: Escherichia coli BL21(DE3)

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