Function and Biology

cryo-EM structure of DOT1L bound to H2B ubiquitinated nucleosome

Source organisms:
Biochemical function: protein heterodimerization activity
Biological process: regulation of cell cycle
Cellular component: nucleosome

EC 2.1.1.360: [Histone H3]-lysine(79) N-trimethyltransferase

Reaction catalysed:
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(79) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(79)
Systematic name:
S-adenosyl-L-methionine:[histone H3]-L-lysine(79) N(6)-trimethyltransferase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF00125
Domain description: Core histone H2A/H2B/H3/H4
Occurring in:
  1. Histone H3.2
  2. Histone H2A
  3. Histone H2B 1.1
The deposited structure of PDB entry 6jma contains 2 copies of Pfam domain PF00125 (Core histone H2A/H2B/H3/H4) in Histone H3.2. Showing 1 copy in chain C [auth A].
The deposited structure of PDB entry 6jma contains 2 copies of Pfam domain PF00125 (Core histone H2A/H2B/H3/H4) in Histone H2A. Showing 1 copy in chain E [auth C].
The deposited structure of PDB entry 6jma contains 2 copies of Pfam domain PF00125 (Core histone H2A/H2B/H3/H4) in Histone H2B 1.1. Showing 1 copy in chain F [auth D].

PF16211
Domain description: C-terminus of histone H2A
Occurring in:
  1. Histone H2A
The deposited structure of PDB entry 6jma contains 2 copies of Pfam domain PF16211 (C-terminus of histone H2A) in Histone H2A. Showing 1 copy in chain E [auth C].

PF15511
Domain description: Centromere kinetochore component CENP-T histone fold
Occurring in:
  1. Histone H4
The deposited structure of PDB entry 6jma contains 2 copies of Pfam domain PF15511 (Centromere kinetochore component CENP-T histone fold) in Histone H4. Showing 1 copy in chain H [auth F].

PF00240
Domain description: Ubiquitin family
Occurring in:
  1. Ubiquitin
The deposited structure of PDB entry 6jma contains 1 copy of Pfam domain PF00240 (Ubiquitin family) in Ubiquitin. Showing 1 copy in chain L [auth Y].

PF08123
Domain description: Histone methylation protein DOT1
Occurring in:
  1. Histone-lysine N-methyltransferase, H3 lysine-79 specific
The deposited structure of PDB entry 6jma contains 1 copy of Pfam domain PF08123 (Histone methylation protein DOT1 ) in Histone-lysine N-methyltransferase, H3 lysine-79 specific. Showing 1 copy in chain K [auth X].

InterPro InterPro annotations
IPR009072
Domain description: Histone-fold
Occurring in:
  1. Histone H3.2
  2. Histone H4
  3. Histone H2A
  4. Histone H2B 1.1
IPR000558
Domain description: Histone H2B
Occurring in:
  1. Histone H2B 1.1
IPR002119
Domain description: Histone H2A
Occurring in:
  1. Histone H2A
IPR001951
Domain description: Histone H4
Occurring in:
  1. Histone H4
IPR000164
Domain description: Histone H3/CENP-A
Occurring in:
  1. Histone H3.2
IPR007125
Domain description: Histone H2A/H2B/H3
Occurring in:
  1. Histone H3.2
  2. Histone H2A
  3. Histone H2B 1.1
IPR019956
Domain description: Ubiquitin domain
Occurring in:
  1. Ubiquitin
IPR000626
Domain description: Ubiquitin-like domain
Occurring in:
  1. Ubiquitin
IPR025789
Domain description: Histone-lysine N-methyltransferase DOT1 domain
Occurring in:
  1. Histone-lysine N-methyltransferase, H3 lysine-79 specific
IPR029063
Domain description: S-adenosyl-L-methionine-dependent methyltransferase superfamily
Occurring in:
  1. Histone-lysine N-methyltransferase, H3 lysine-79 specific
IPR032454
Domain description: Histone H2A, C-terminal domain
Occurring in:
  1. Histone H2A
IPR032458
Domain description: Histone H2A conserved site
Occurring in:
  1. Histone H2A
IPR035425
Domain description: CENP-T/Histone H4, histone fold
Occurring in:
  1. Histone H4
IPR004823
Domain description: TATA box binding protein associated factor (TAF), histone-like fold domain
Occurring in:
  1. Histone H4
IPR019954
Domain description: Ubiquitin conserved site
Occurring in:
  1. Ubiquitin
IPR029071
Domain description: Ubiquitin-like domain superfamily
Occurring in:
  1. Ubiquitin
IPR030445
Domain description: Histone H3-K79 methyltransferase
Occurring in:
  1. Histone-lysine N-methyltransferase, H3 lysine-79 specific