› 6jpk

X-ray diffraction
2.1Å resolution

Crystal structure of S. pombe aspartate aminotransferase

Released:
DOI: 10.2210/pdb6jpk/pdb
PDB entry 6jpk coloured by chain, front view.
PDB entry 6jpk coloured by chain, side view.
PDB entry 6jpk coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Crystal structure of L-aspartate aminotransferase from Schizosaccharomyces pombe.
Jeong SY, Jin H, Chang JH
OpenAccess logo PLoS One 14 e0221975 (2019)
PMID: 31465495

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase, cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 47.28 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O42652 (Residues: 1-409; Coverage: 100%)
Gene names: SPAC10F6.13c, aat2
Sequence domains: Aminotransferase class I and II

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P21
Unit cell:
a: 62.133Å b: 53.111Å c: 130.263Å
α: 90° β: 96.7° γ: 90°
R-values:
R R work R free
0.144 0.143 0.183
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Aspartate Transaminase AST2 Involved in Sporulation and Necrotrophic Pathogenesis in the Hemibiotrophs Magnaporthe oryzae and Colletotrichum graminicola.
Zhang et al. (2022)
2 more

1 mention without citation

Crystal structure of L-aspartate aminotransferase from Schizosaccharomyces pombe.
Jeong et al. (2019)