› 6jqh

X-ray diffraction
2.3Å resolution

Crystal structure of MaDA

Released:
DOI: 10.2210/pdb6jqh/pdb
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PDB entry 6jqh coloured by chain, front view.
PDB entry 6jqh coloured by chain, side view.
PDB entry 6jqh coloured by chain, top view.
Source organism: Morus alba
Primary publication:
FAD-dependent enzyme-catalysed intermolecular [4+2] cycloaddition in natural product biosynthesis.
Gao L, Su C, Du X, Wang R, Chen S, Zhou Y, Liu C, Liu X, Tian R, Zhang L, Xie K, Chen S, Guo Q, Guo L, Hano Y, Shimazaki M, Minami A, Oikawa H, Huang N, Houk KN, Huang L, Dai J, Lei X
Nat Chem 12 620-628 (2020)
PMID: 32451436

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166945 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MaDA Chains: A, B
Molecule details ›
Chains: A, B
Length: 517 amino acids
Theoretical weight: 58.49 KDa
Source organism: Morus alba
Expression system: Trichoplusia ni

Ligands and Environments

1 bound ligand:
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P21
Unit cell:
a: 74.665Å b: 115.381Å c: 95.87Å
α: 90° β: 96.23° γ: 90°
R-values:
R R work R free
0.187 0.186 0.226
Expression system: Trichoplusia ni

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Citations

16 review citations

Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells.
Zhu et al. (2021)
15 more

2 mentions without citation

Chemo-enzymatic total synthesis: current approaches toward the integration of chemical and enzymatic transformations.
Tanifuji et al. (2024)
1 more

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