› 6k3b

X-ray diffraction
1.97Å resolution

Crystal structure of Lpg2147-Lpg2149 complex

Released:
DOI: 10.2210/pdb6k3b/pdb
PDB entry 6k3b coloured by chain, front view.
PDB entry 6k3b coloured by chain, side view.
PDB entry 6k3b coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
Structural insights into the mechanism and inhibition of transglutaminase-induced ubiquitination by the Legionella effector MavC.
Mu Y, Wang Y, Huang Y, Li D, Han Y, Chang M, Fu J, Xie Y, Ren J, Wang H, Zhang Y, Luo ZQ, Feng Y
OpenAccess logo Nat Commun 11 1774 (2020)
PMID: 32286321

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178628 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MvcA insertion domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 382 amino acids
Theoretical weight: 43.53 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZTL4 (Residues: 7-384; Coverage: 78%)
Gene name: lpg2147
Sequence domains: MvcA insertion domain
Uncharacterized protein Chain: B
Molecule details ›
Chain: B
Length: 108 amino acids
Theoretical weight: 12.79 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZTL2 (Residues: 11-114; Coverage: 87%)
Gene name: lpg2149

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 52.872Å b: 83.781Å c: 124.182Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.205
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Divergence of Legionella Effectors Reversing Conventional and Unconventional Ubiquitination.
Kitao et al. (2020)
6 more

1 mention without citation

The unity of opposites: Strategic interplay between bacterial effectors to regulate cellular homeostasis.
Iyer et al. (2021)