› 6k4d

X-ray diffraction
1.7Å resolution

Ancestral luciferase AncLamp in complex with ATP and D-luciferin

Released:
DOI: 10.2210/pdb6k4d/pdb
PDB entry 6k4d coloured by chain, front view.
PDB entry 6k4d coloured by chain, side view.
PDB entry 6k4d coloured by chain, top view.
Source organism: Lampyridae
Primary publication:
Resurrecting the ancient glow of the fireflies.
Oba Y, Konishi K, Yano D, Shibata H, Kato D, Shirai T
OpenAccess logo Sci Adv 6 (2020)
PMID: 33268373

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ancestral luciferase AncLamp Chain: A
Molecule details ›
Chain: A
Length: 545 amino acids
Theoretical weight: 60.37 KDa
Source organism: Lampyridae
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand D4F 1 x D4F
Ligand ESJ 1 x ESJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2221
Unit cell:
a: 48.76Å b: 123.68Å c: 177.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.188
Expression system: Escherichia coli

Quick links

Citations

1 review citation

How to Select Firefly Luciferin Analogues for In Vivo Imaging.
Saito-Moriya et al. (2021)
6 other articles

1 mention without citation

Resurrecting the ancient glow of the fireflies.
Oba et al. (2020)