› 6k5d

X-ray diffraction
3.2Å resolution

Crystal structure of the E148N mutant CLC-ec1 in presence of 200 mM NaBr

Released:
DOI: 10.2210/pdb6k5d/pdb
Model geometry
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PDB entry 6k5d coloured by chain, front view.
PDB entry 6k5d coloured by chain, side view.
PDB entry 6k5d coloured by chain, top view.
Source organisms:
Primary publication:
Mutation of external glutamate residue reveals a new intermediate transport state and anion binding site in a CLC Cl-/H+ antiporter.
Park K, Lee BC, Lim HH
Proc Natl Acad Sci U S A 116 17345-17354 (2019)
PMID: 31409705

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211182 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H(+)/Cl(-) exchange transporter ClcA Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 50.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37019 (Residues: 1-473; Coverage: 100%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Fab fragment, heavy chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 222 amino acids
Theoretical weight: 23.82 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Fab fragment, light chain Chains: D, F

Ligands and Environments

1 bound ligand:
Ligand BR 8 x BR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: C2
Unit cell:
a: 231.719Å b: 99.324Å c: 171.465Å
α: 90° β: 131.92° γ: 90°
R-values:
R R work R free
0.217 0.215 0.262
Expression systems:
  • Escherichia coli
  • Mus musculus

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Citations

1 review citation

The challenges and prospects of Escherichia coli as an organic acid production host under acid stress.
Yang et al. (2021)
6 other articles

2 mentions without citation

Virtual screening by targeting proteolytic sites of furin and TMPRSS2 to propose potential compounds obstructing the entry of SARS-CoV-2 virus into human host cells.
Vardhan et al. (2022)
1 more

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