› 6k73

X-ray diffraction
2.77Å resolution

Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis

Released:
DOI: 10.2210/pdb6k73/pdb
PDB entry 6k73 coloured by chain, front view.
PDB entry 6k73 coloured by chain, side view.
PDB entry 6k73 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Chaperone-tip adhesin complex is vital for synergistic activation of CFA/I fimbriae biogenesis.
He LH, Wang H, Liu Y, Kang M, Li T, Li CC, Tong AP, Zhu YB, Song YJ, Savarino SJ, Prouty MG, Xia D, Bao R
OpenAccess logo PLoS Pathog 16 e1008848 (2020)
PMID: 33007034

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-279878 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colonization factor antigen I chaperone CfaA Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
CFA/I fimbrial subunit E Chains: C, D
Molecule details ›
Chains: C, D
Length: 341 amino acids
Theoretical weight: 37.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25734 (Residues: 24-360; Coverage: 94%)
Gene name: cfaE
Sequence domains: CblD like pilus biogenesis initiator

Ligands and Environments

2 bound ligands:
Ligand GOL 4 x GOL
Ligand NI 4 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: R32
Unit cell:
a: 217.158Å b: 217.158Å c: 177.091Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.225 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Stochastic chain termination in bacterial pilus assembly.
Giese et al. (2023)
1 more

2 mentions without citation

Large-Scale Discovery of Microbial Fibrillar Adhesins and Identification of Novel Members of Adhesive Domain Families.
Monzon et al. (2022)
1 more