› 6k89

X-ray diffraction
1.69Å resolution

The closed state of RGLG1 VWA domain

Released:
DOI: 10.2210/pdb6k89/pdb
PDB entry 6k89 coloured by chain, front view.
PDB entry 6k89 coloured by chain, side view.
PDB entry 6k89 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Wang Q, Wu Y

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193518 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase RGLG1 Chain: B
Molecule details ›
Chain: B
Length: 301 amino acids
Theoretical weight: 33.17 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SS90 (Residues: 129-419; Coverage: 60%)
Gene names: At3g01650, F4P13.19, RGLG1
Sequence domains: Copine

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AichiSR BEAMLINE BL2S1
Spacegroup: P212121
Unit cell:
a: 45.619Å b: 72.935Å c: 80.077Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.231
Expression system: Escherichia coli

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