› 6kfc

X-ray diffraction
2.1Å resolution

Hydroxynitrile lyase from the millipede, Chamberlinius hualienensis, complexed with cyanide ion

Released:
DOI: 10.2210/pdb6kfc/pdb
PDB entry 6kfc coloured by chain, front view.
PDB entry 6kfc coloured by chain, side view.
PDB entry 6kfc coloured by chain, top view.
Source organism: Chamberlinius hualienensis
Primary publication:
R-hydroxynitrile lyase from the cyanogenic millipede, Chamberlinius hualienensis-A new entry to the carrier protein family Lipocalines.
Motojima F, Izumi A, Nuylert A, Zhai Z, Dadashipour M, Shichida S, Yamaguchi T, Nakano S, Asano Y
OpenAccess logo FEBS J 288 1679-1695 (2021)
PMID: 32679618

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Hydroxynitrile lyase Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.24 KDa
Source organism: Chamberlinius hualienensis
UniProt:
  • Canonical: A0A0H5BR52 (Residues: 22-183; Coverage: 100%)
Gene name: ChuaHNL

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand CYN 1 x CYN
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P6122
Unit cell:
a: 58.59Å b: 58.59Å c: 226.233Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.187 0.232

Quick links

Citations

3 citation in other articles

Discovery and Structural Analysis to Improve the Enantioselectivity of Hydroxynitrile Lyase from Parafontaria laminata Millipedes for (R)-2-Chloromandelonitrile Synthesis.
Nuylert et al. (2020)
2 more

1 mention without citation

R-hydroxynitrile lyase from the cyanogenic millipede, Chamberlinius hualienensis-A new entry to the carrier protein family Lipocalines.
Motojima et al. (2021)