› 6kng

Electron Microscopy
2.85Å resolution

CryoEM map and model of Nitrite Reductase at pH 8.1

Released:
DOI: 10.2210/pdb6kng/pdb
PDB entry 6kng coloured by chain, front view.
PDB entry 6kng coloured by chain, side view.
PDB entry 6kng coloured by chain, top view.
Source organism: Achromobacter cycloclastes
Primary publication:
2.85 and 2.99 Å resolution structures of 110 kDa nitrite reductase determined by 200 kV cryogenic electron microscopy.
Adachi N, Yamaguchi T, Moriya T, Kawasaki M, Koiwai K, Shinoda A, Yamada Y, Yumoto F, Kohzuma T, Senda T
J Struct Biol 213 107768 (2021)
PMID: 34217801
Related structures: EMD-0731

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 334 amino acids
Theoretical weight: 36.62 KDa
Source organism: Achromobacter cycloclastes
UniProt:
  • Canonical: P25006 (Residues: 45-378; Coverage: 98%)
Gene name: nirK
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CU 6 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.85Å
Relevant EMDB volumes: EMD-0731

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