6knu

X-ray diffraction
2.7Å resolution

THRb mutation with a novel agonist

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-145483 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thyroid hormone receptor beta Chain: A
Molecule details ›
Chain: A
Length: 250 amino acids
Theoretical weight: 28.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10828 (Residues: 211-460; Coverage: 54%)
Gene names: ERBA2, NR1A2, THR1, THRB
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Nuclear receptor coactivator 2 Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15596 (Residues: 741-751; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P43212
Unit cell:
a: 109.834Å b: 109.834Å c: 57.524Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.231
Expression system: Escherichia coli BL21(DE3)