› 6kos

X-ray diffraction
2Å resolution

Crystal structure of SUWA (Super WA20), a hyper-stable de novo protein with a dimeric bisecting topology

Released:
DOI: 10.2210/pdb6kos/pdb
PDB entry 6kos coloured by chain, front view.
PDB entry 6kos coloured by chain, side view.
PDB entry 6kos coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Hyperstable De Novo Protein with a Dimeric Bisecting Topology.
Kimura N, Mochizuki K, Umezawa K, Hecht MH, Arai R
ACS Synth Biol 9 254-259 (2020)
PMID: 31951376

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SUWA (Super WA20) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 102 amino acids
Theoretical weight: 12.54 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 65.636Å b: 71.93Å c: 81.563Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Recent Progress Using De Novo Design to Study Protein Structure, Design and Binding Interactions.
Ferrando et al. (2021)
5 other articles

1 mention without citation

Rational thermostabilisation of four-helix bundle dimeric de novo proteins.
Irumagawa et al. (2021)