› 6krn

X-ray diffraction
1.65Å resolution

Crystal structure of GH30 xylanase B from Talaromyces cellulolyticus expressed by Pichia pastoris in complex with aldotriuronic acid

Released:
DOI: 10.2210/pdb6krn/pdb
PDB entry 6krn coloured by chain, front view.
PDB entry 6krn coloured by chain, side view.
PDB entry 6krn coloured by chain, top view.
Source organism: Talaromyces cellulolyticus CF-2612
Primary publication:
Substrate recognition by a bifunctional GH30-7 xylanase B from Talaromyces cellulolyticus.
Nakamichi Y, Watanabe M, Matsushika A, Inoue H
OpenAccess logo FEBS Open Bio 10 1180-1189 (2020)
PMID: 32359208

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-497957 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Bifunctional glucuronoxylanase and xylobiohydrolase Xyn30B Chain: A
Molecule details ›
Chain: A
Length: 545 amino acids
Theoretical weight: 59.3 KDa
Source organism: Talaromyces cellulolyticus CF-2612
Expression system: Komagataella phaffii GS115
UniProt:
  • Canonical: A0A510NXC4 (Residues: 23-474; Coverage: 100%)
Gene name: TCE0_018f06110
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: XYP, GCV
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 63.251Å b: 78.7Å c: 118.418Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.19
Expression system: Komagataella phaffii GS115

Quick links

Citations

6 citation in other articles

A novel bacterial GH30 xylobiohydrolase from Hungateiclostridium clariflavum.
Šuchová et al. (2021)
5 more

2 mentions without citation

Substrate recognition by a bifunctional GH30-7 xylanase B from Talaromyces cellulolyticus.
Nakamichi et al. (2020)
1 more