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Structural basis for domain rotation during adenylation of active site K123 and fragment library screening against NAD+ -dependent DNA ligase from Mycobacterium tuberculosis

Source organism: Mycobacterium tuberculosis H37Rv
Biochemical function: DNA ligase (NAD+) activity
Biological process: not assigned
Cellular component: not assigned

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EC 6.5.1.2: DNA ligase (NAD(+))

Reaction catalysed:
(1a) NAD(+) + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide
Systematic name:
Poly(deoxyribonucleotide)-3'-hydroxyl:5'-phospho-poly(deoxyribonucleotide) ligase (NAD(+))
Alternative Name(s):
  • DNA joinase
  • DNA ligase
  • DNA repair enzyme
  • DNA-joining enzyme
  • Deoxyribonucleate ligase
  • Deoxyribonucleic acid joinase
  • Deoxyribonucleic acid ligase
  • Deoxyribonucleic joinase
  • Deoxyribonucleic ligase
  • Deoxyribonucleic repair enzyme
  • Deoxyribonucleic-joining enzyme
  • Poly(deoxyribonucleotide):poly(deoxyribonucleotide) ligase (AMP-forming, NMN-forming)
  • Polydeoxyribonucleotide synthase (NAD(+))
  • Polydeoxyribonucleotide synthase (NAD+)
  • Polynucleotide ligase
  • Polynucleotide ligase (NAD(+))
  • Polynucleotide ligase (NAD+)
  • Polynucleotide ligase (nicotinamide adenine dinucleotide)
  • Polynucleotide synthetase
  • Polynucleotide synthetase (nicotinamide adenine dinucleotide)

GO terms

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF01653
Domain description: NAD-dependent DNA ligase adenylation domain
Occurring in:
  1. DNA ligase A
The deposited structure of PDB entry 6ksc contains 1 copy of Pfam domain PF01653 (NAD-dependent DNA ligase adenylation domain) in DNA ligase A. Showing 1 copy in chain A.

PF22745
Domain description: DNA ligase-like, N-terminal NAD+-binding domain
Occurring in:
  1. DNA ligase A

InterPro InterPro annotations
IPR013839
Domain description: NAD-dependent DNA ligase, adenylation
Occurring in:
  1. DNA ligase A
IPR013840
Domain description: NAD-dependent DNA ligase, N-terminal
Occurring in:
  1. DNA ligase A

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