› 6l48

Electron Microscopy
3.5Å resolution

Structure of the human sterol O-acyltransferase 1 in resting state

Released:
DOI: 10.2210/pdb6l48/pdb
PDB entry 6l48 coloured by chain, front view.
PDB entry 6l48 coloured by chain, side view.
PDB entry 6l48 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the inhibition mechanism of human sterol O-acyltransferase 1 by a competitive inhibitor.
Guan C, Niu Y, Chen SC, Kang Y, Wu JX, Nishi K, Chang CCY, Chang TY, Luo T, Chen L
Nat Commun 11 2478 (2020)
PMID: 32424158
Related structures: EMD-0832

Function and Biology Details

Reaction catalysed: 
A long-chain acyl-CoA + a sterol = CoA + a long-chain 3-hydroxysterol ester
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152963 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sterol O-acyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 485 amino acids
Theoretical weight: 57.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P35610 (Residues: 66-550; Coverage: 88%)
Gene names: ACACT, ACACT1, ACAT, ACAT1, SOAT, SOAT1, STAT
Sequence domains: MBOAT, membrane-bound O-acyltransferase family

Ligands and Environments

1 bound ligand:
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-0832
Expression system: Homo sapiens

Quick links

Citations

10 review citations

Update and nomenclature proposal for mammalian lysophospholipid acyltransferases, which create membrane phospholipid diversity.
Valentine et al. (2022)
9 more

2 mentions without citation

Ghrelin octanoylation by ghrelin O-acyltransferase: protein acylation impacting metabolic and neuroendocrine signalling.
Davis et al. (2021)
1 more