› 6lbl

X-ray diffraction
1.68Å resolution

Crystal structure of IMP-1 metallo-beta-lactamase in complex with NO9 inhibitor

Released:
DOI: 10.2210/pdb6lbl/pdb
PDB entry 6lbl coloured by chain, front view.
PDB entry 6lbl coloured by chain, side view.
PDB entry 6lbl coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Sulfamoyl Heteroarylcarboxylic Acids as Promising Metallo-β-Lactamase Inhibitors for Controlling Bacterial Carbapenem Resistance.
Wachino JI, Jin W, Kimura K, Kurosaki H, Sato A, Arakawa Y
OpenAccess logo mBio 11 (2020)
PMID: 32184250

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase IMP-1 Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 25.15 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52699 (Residues: 19-246; Coverage: 100%)
Sequence domains: Metallo-beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand NA 1 x NA
Ligand NO9 1 x NO9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AichiSR BEAMLINE BL2S1
Spacegroup: P212121
Unit cell:
a: 35.92Å b: 56.61Å c: 100.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.208
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Recent Developments to Cope the Antibacterial Resistance via β-Lactamase Inhibition.
Iqbal et al. (2022)
3 more

2 mentions without citation

Sulfamoyl Heteroarylcarboxylic Acids as Promising Metallo-β-Lactamase Inhibitors for Controlling Bacterial Carbapenem Resistance.
Wachino et al. (2020)
1 more