› 6ldt

X-ray diffraction
1.93Å resolution

K245A mutant of L-tyrosine decarboxylase from Methanocaldococcus jannaschii complexed with a post-decarboxylation quinonoid-like intermediate formed with L-tyrosine

Released:
DOI: 10.2210/pdb6ldt/pdb
PDB entry 6ldt coloured by chain, front view.
PDB entry 6ldt coloured by chain, side view.
PDB entry 6ldt coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii DSM 2661
Primary publication:
Structural insights into the mechanism of internal aldimine formation and catalytic loop dynamics in an archaeal Group II decarboxylase.
Chellam Gayathri S, Manoj N
J Struct Biol 208 137-151 (2019)
PMID: 31445086

Function and Biology Details

Reactions catalysed: 
L-aspartate = beta-alanine + CO(2)
L-tyrosine = tyramine + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178771 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-tyrosine/L-aspartate decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 47.6 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q60358 (Residues: 1-396; Coverage: 100%)
Gene names: MJ0050, mfnA
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain

Ligands and Environments

3 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 1 x SO4
Ligand EBR 1 x EBR
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P43212
Unit cell:
a: 98.37Å b: 98.37Å c: 121.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.227
Expression system: Escherichia coli B

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