› 6lfg

Electron Microscopy
9.58Å resolution

Cryo-EM structure of the Drosophila CTP synthase product-bound filament

Released:
DOI: 10.2210/pdb6lfg/pdb
PDB entry 6lfg coloured by chain, front view.
PDB entry 6lfg coloured by chain, side view.
PDB entry 6lfg coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Drosophila CTP synthase can form distinct substrate- and product-bound filaments.
Zhou X, Guo CJ, Hu HH, Zhong J, Sun Q, Liu D, Zhou S, Chang CC, Liu JL
J Genet Genomics 46 537-545 (2019)
PMID: 31902586
Related structures: EMD-0876

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194308 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CTP synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 562 amino acids
Theoretical weight: 63.12 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9VUL1 (Residues: 1-562; Coverage: 90%)
Gene names: CG45070, CTPsyn, Ctps
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.58Å
Relevant EMDB volumes: EMD-0876
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

3 review citations

CTP synthase: the hissing of the cellular serpent.
Thangadurai et al. (2022)
2 more

2 mentions without citation

Tunnel Architectures in Enzyme Systems that Transport Gaseous Substrates.
Singh et al. (2021)
1 more