› 6lfj

X-ray diffraction
1.84Å resolution

Crystal structure of mouse DCAR2 CRD domain complex with IPM2

Released:
DOI: 10.2210/pdb6lfj/pdb
PDB entry 6lfj coloured by chain, front view.
PDB entry 6lfj coloured by chain, side view.
PDB entry 6lfj coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural insight into the recognition of pathogen-derived phosphoglycolipids by C-type lectin receptor DCAR.
Omahdi Z, Horikawa Y, Nagae M, Toyonaga K, Imamura A, Takato K, Teramoto T, Ishida H, Kakuta Y, Yamasaki S
J Biol Chem 295 5807-5817 (2020)
PMID: 32139512

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-type lectin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 15.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9D8Q7 (Residues: 45-176; Coverage: 75%)
Gene names: Clec4b, Clec4b1, Dcar
Sequence domains: Lectin C-type domain

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand B3P 1 x B3P
Ligand MAN 3 x MAN
Ligand IPD 2 x IPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P212121
Unit cell:
a: 65.689Å b: 72.564Å c: 100.469Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.198
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Chemical Synthesis of Cell Wall Constituents of Mycobacterium tuberculosis.
Holzheimer et al. (2021)
4 more

2 mentions without citation

3D Structural View of Pathogen Recognition by Mammalian Lectin Receptors.
Manabe et al. (2021)
1 more