› 6lhy

X-ray diffraction
1.8Å resolution

Crystal structure of ThsB

Released:
DOI: 10.2210/pdb6lhy/pdb
PDB entry 6lhy coloured by chain, front view.
PDB entry 6lhy coloured by chain, side view.
PDB entry 6lhy coloured by chain, top view.
Source organism: Bacillus cereus MSX-D12
Primary publication:
Structural and functional evidence of bacterial antiphage protection by Thoeris defense system via NAD+ degradation.
Ka D, Oh H, Park E, Kim JH, Bae E
OpenAccess logo Nat Commun 11 2816 (2020)
PMID: 32499527

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125385 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative cyclic ADP-D-ribose synthase ThsB1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 22.64 KDa
Source organism: Bacillus cereus MSX-D12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: J8G8J6 (Residues: 1-192; Coverage: 100%)
Gene names: II9_05449, thsB, thsB1
Sequence domains: Thoeris protein ThsB, TIR-like domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 53.57Å b: 67.731Å c: 59.169Å
α: 90° β: 92.38° γ: 90°
R-values:
R R work R free
0.192 0.191 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Mechanisms and clinical importance of bacteriophage resistance.
Egido et al. (2022)
6 more

2 mentions without citation

Cryo-EM structure of an active bacterial TIR-STING filament complex.
Morehouse et al. (2022)
1 more