› 6li1

X-ray diffraction
2.9Å resolution

Crystal structure of GPR52 ligand free form with flavodoxin fusion

Released:
DOI: 10.2210/pdb6li1/pdb
PDB entry 6li1 coloured by chain, front view.
PDB entry 6li1 coloured by chain, side view.
PDB entry 6li1 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of ligand recognition and self-activation of orphan GPR52.
Lin X, Li M, Wang N, Wu Y, Luo Z, Guo S, Han GW, Li S, Yue Y, Wei X, Xie X, Chen Y, Zhao S, Wu J, Lei M, Xu F
Nature 579 152-157 (2020)
PMID: 32076264

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132398 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavodoxin; G-protein coupled receptor 52 Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 50.16 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y2T5 (Residues: 17-236, 265-341; Coverage: 82%)
  • Canonical: P00323 (Residues: 3-148; Coverage: 99%)
Gene names: DVU_2680, GPR52
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand OLC 2 x OLC
Ligand FMN 1 x FMN
Ligand PEG 1 x PEG
Ligand PGE 1 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 66.648Å b: 79.891Å c: 148.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.244 0.267
Expression system: Spodoptera frugiperda

Quick links

Citations

16 review citations

Cryo-electron microscopy analysis of small membrane proteins.
Nygaard et al. (2020)
15 more

8 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
7 more