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Cryo-EM structure of 90S small ribosomal subunit precursors in transition states.

Du Y, An W, Zhu X, Sun Q, Qi J, Ye K
Science 369 1477-1481 (2020)
Related entries: 6lqp, 6lqq, 6lqr, 6lqs, 6lqt, 6lqv

Cited: 38 times
EuropePMC logo PMID: 32943522

Abstract

The 90S preribosome is a large, early assembly intermediate of small ribosomal subunits that undergoes structural changes to give a pre-40S ribosome. Here, we gained insight into this transition by determining cryo-electron microscopy structures of Saccharomyces cerevisiae intermediates in the path from the 90S to the pre-40S The full transition is blocked by deletion of RNA helicase Dhr1. A series of structural snapshots revealed that the excised 5' external transcribed spacer (5' ETS) is degraded within 90S, driving stepwise disassembly of assembly factors and ribosome maturation. The nuclear exosome, an RNA degradation machine, docks on the 90S through helicase Mtr4 and is primed to digest the 3' end of the 5' ETS. The structures resolved between 3.2- and 8.6-angstrom resolution reveal key intermediates and the critical role of 5' ETS degradation in 90S progression.

Articles - 6lqu mentioned but not cited (2)

  1. Additional principles that govern the release of pre-ribosomes from the nucleolus into the nucleoplasm in yeast. LaPeruta AJ, Micic J, Woolford JL. Nucleic Acids Res 51 10867-10883 (2023)
  2. Artificial intelligence-assisted cryoEM structure of Bfr2-Lcp5 complex observed in the yeast small subunit processome. Zhao Y, Rai J, Xu C, He H, Li H. Commun Biol 5 523 (2022)


Reviews citing this publication (8)

  1. RNA folding and functions of RNA helicases in ribosome biogenesis. Mitterer V, Pertschy B. RNA Biol 19 781-810 (2022)
  2. The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches. Gerovac M, Vogel J, Smirnov A. Front Mol Biosci 8 661448 (2021)
  3. Ribosome biogenesis factors-from names to functions. Dörner K, Ruggeri C, Zemp I, Kutay U. EMBO J 42 e112699 (2023)
  4. Cellular functions of eukaryotic RNA helicases and their links to human diseases. Bohnsack KE, Yi S, Venus S, Jankowsky E, Bohnsack MT. Nat Rev Mol Cell Biol 24 749-769 (2023)
  5. Quality control ensures fidelity in ribosome assembly and cellular health. Parker MD, Karbstein K. J Cell Biol 222 e202209115 (2023)
  6. Genetics animates structure: leveraging genetic interactions to study the dynamics of ribosome biogenesis. Black JJ, Johnson AW. Curr Genet 67 729-738 (2021)
  7. Mtr4 RNA helicase structures and interactions. Olsen KJ, Johnson SJ. Biol Chem 402 605-616 (2021)
  8. Ribosome biogenesis and function in development and disease. Ni C, Buszczak M. Development 150 dev201187 (2023)

Articles citing this publication (28)

  1. Nucleolar maturation of the human small subunit processome. Singh S, Vanden Broeck A, Miller L, Chaker-Margot M, Klinge S. Science 373 eabj5338 (2021)
  2. In situ cryo-electron tomography reveals gradient organization of ribosome biogenesis in intact nucleoli. Erdmann PS, Hou Z, Klumpe S, Khavnekar S, Beck F, Wilfling F, Plitzko JM, Baumeister W. Nat Commun 12 5364 (2021)
  3. Mechanism of mitoribosomal small subunit biogenesis and preinitiation. Itoh Y, Khawaja A, Laptev I, Cipullo M, Atanassov I, Sergiev P, Rorbach J, Amunts A. Nature 606 603-608 (2022)
  4. Assembly factors chaperone ribosomal RNA folding by isolating helical junctions that are prone to misfolding. Huang H, Karbstein K. Proc Natl Acad Sci U S A 118 e2101164118 (2021)
  5. Bud23 promotes the final disassembly of the small subunit Processome in Saccharomyces cerevisiae. Black JJ, Sardana R, Elmir EW, Johnson AW. PLoS Genet 16 e1009215 (2020)
  6. The nucleolus as a polarized coaxial cable in which the rDNA axis is surrounded by dynamic subunit-specific phases. Tartakoff AM, Chen L, Raghavachari S, Gitiforooz D, Dhinakaran A, Ni CL, Pasadyn C, Mahabeleshwar GH, Pasadyn V, Woolford JL. Curr Biol 31 2507-2519.e4 (2021)
  7. Prp43/DHX15 exemplify RNA helicase multifunctionality in the gene expression network. Bohnsack KE, Kanwal N, Bohnsack MT. Nucleic Acids Res 50 9012-9022 (2022)
  8. The nucleoplasmic phase of pre-40S formation prior to nuclear export. Cheng J, Lau B, Thoms M, Ameismeier M, Berninghausen O, Hurt E, Beckmann R. Nucleic Acids Res 50 11924-11937 (2022)
  9. Release of the ribosome biogenesis factor Bud23 from small subunit precursors in yeast. Black JJ, Johnson AW. RNA 28 371-389 (2022)
  10. The modifying enzyme Tsr3 establishes the hierarchy of Rio kinase binding in 40S ribosome assembly. Huang H, Parker M, Karbstein K. RNA 28 568-582 (2022)
  11. Efficient fractionation and analysis of ribosome assembly intermediates in human cells. Nieto B, Gaspar SG, Sapio RT, Clavaín L, Bustelo XR, Pestov DG, Dosil M. RNA Biol 18 182-197 (2021)
  12. Hydrogen-deuterium exchange mass spectrometry of Mtr4 with diverse RNAs reveals substrate-dependent dynamics and interfaces in the arch. Zhang N, Olsen KJ, Ball D, Johnson SJ, D'Arcy S. Nucleic Acids Res 50 4042-4053 (2022)
  13. Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S biogenesis. Lau B, Huang Z, Kellner N, Niu S, Berninghausen O, Beckmann R, Hurt E, Cheng J. EMBO Rep 24 e56910 (2023)
  14. Using DMS-MaPseq to uncover the roles of DEAD-box proteins in ribosome assembly. Liu X, Huang H, Karbstein K. Methods 204 249-257 (2022)
  15. Arabidopsis HOT3/eIF5B1 constrains rRNA RNAi by facilitating 18S rRNA maturation. Hang R, Xu Y, Wang X, Hu H, Flynn N, You C, Chen X. Proc Natl Acad Sci U S A 120 e2301081120 (2023)
  16. Attacking a DEAD problem: The role of DEAD-box ATPases in ribosome assembly and beyond. Karbstein K. Methods Enzymol 673 19-38 (2022)
  17. Concurrent remodelling of nucleolar 60S subunit precursors by the Rea1 ATPase and Spb4 RNA helicase. Mitterer V, Thoms M, Buschauer R, Berninghausen O, Hurt E, Beckmann R. Elife 12 e84877 (2023)
  18. Ileum tissue single-cell mRNA sequencing elucidates the cellular architecture of pathophysiological changes associated with weaning in piglets. Tang W, Zhong Y, Wei Y, Deng Z, Mao J, Liu J, Valencak TG, Liu J, Xu H, Wang H. BMC Biol 20 123 (2022)
  19. The recognition mode between hsRBFA and mitoribosome 12S rRNA during mitoribosomal biogenesis. Zhou W, Liu X, Lv M, Shi Y, Zhang L. Nucleic Acids Res 51 1353-1363 (2023)
  20. Trehalase Inhibitor Validamycin May Have Additional Mechanisms of Toxicology against Rhizoctonia cerealis. Yang X, Shu Y, Cao S, Sun H, Zhang X, Zhang A, Li Y, Ma D, Chen H, Li W. J Fungi (Basel) 9 846 (2023)
  21. Cms1 coordinates stepwise local 90S pre-ribosome assembly with timely snR83 release. Lau B, Beine-Golovchuk O, Kornprobst M, Cheng J, Kressler D, Jády B, Kiss T, Beckmann R, Hurt E. Cell Rep 41 111684 (2022)
  22. Dissecting Trypanosoma brucei RRP44 function in the maturation of segmented ribosomal RNA using a regulated genetic complementation system. Guerra-Slompo EP, Cesaro G, Guimarães BG, Zanchin NIT. Nucleic Acids Res 51 396-419 (2023)
  23. Structural insights into coordinating 5S RNP rotation with ITS2 pre-RNA processing during ribosome formation. Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E. EMBO Rep 24 e57984 (2023)
  24. The C-terminal tail of ribosomal protein Rps15 is engaged in cytoplasmic pre-40S maturation. Rössler I, Weigl S, Fernández-Fernández J, Martín-Villanueva S, Strauss D, Hurt E, de la Cruz J, Pertschy B. RNA Biol 19 560-574 (2022)
  25. Visualizing the nucleoplasmic maturation of human pre-60S ribosomal particles. Zhang Y, Liang X, Luo S, Chen Y, Li Y, Ma C, Li N, Gao N. Cell Res 33 867-878 (2023)
  26. A disease associated mutant reveals how Ltv1 orchestrates RP assembly and rRNA folding of the small ribosomal subunit head. Blomqvist EK, Huang H, Karbstein K. PLoS Genet 19 e1010862 (2023)
  27. Impact of the yeast S0/uS2-cluster ribosomal protein rpS21/eS21 on rRNA folding and the architecture of small ribosomal subunit precursors. Pöll G, Griesenbeck J, Tschochner H, Milkereit P. PLoS One 18 e0283698 (2023)
  28. Structural Probing with MNase Tethered to Ribosome Assembly Factors Resolves Flexible RNA Regions within the Nascent Pre-Ribosomal RNA. Dielforder T, Braun CM, Hölzgen F, Li S, Thiele M, Huber M, Ohmayer U, Perez-Fernandez J. Noncoding RNA 8 1 (2022)


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