› 6lth

Electron Microscopy
3Å resolution

Structure of human BAF Base module

Released:
DOI: 10.2210/pdb6lth/pdb
PDB entry 6lth coloured by chain, front view.
PDB entry 6lth coloured by chain, side view.
PDB entry 6lth coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of nucleosome-bound human BAF complex.
He S, Wu Z, Tian Y, Yu Z, Yu J, Wang X, Li J, Liu B, Xu Y
Science 367 875-881 (2020)
PMID: 32001526
Related structures: EMD-0968

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-126984 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Transcription activator BRG1 Chain: I
Molecule details ›
Chain: I
Length: 1647 amino acids
Theoretical weight: 184.92 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P51532 (Residues: 1-1647; Coverage: 100%)
Gene names: BAF190A, BRG1, SMARCA4, SNF2B, SNF2L4
Sequence domains:
AT-rich interactive domain-containing protein 1A Chain: L
Molecule details ›
Chain: L
Length: 2285 amino acids
Theoretical weight: 242.25 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O14497 (Residues: 1-2285; Coverage: 100%)
Gene names: ARID1A, BAF250, BAF250A, C1orf4, OSA1, SMARCF1
Sequence domains:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 Chain: M
Molecule details ›
Chain: M
Length: 385 amino acids
Theoretical weight: 44.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q12824 (Residues: 1-385; Coverage: 100%)
Gene names: BAF47, INI1, SMARCB1, SNF5L1
Sequence domains:
SWI/SNF complex subunit SMARCC2 Chains: N, O
Molecule details ›
Chains: N, O
Length: 1214 amino acids
Theoretical weight: 133.05 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8TAQ2 (Residues: 1-1214; Coverage: 100%)
Gene names: BAF170, SMARCC2
Sequence domains:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 Chain: P
Molecule details ›
Chain: P
Length: 515 amino acids
Theoretical weight: 58.31 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96GM5 (Residues: 1-515; Coverage: 100%)
Gene names: BAF60A, SMARCD1
Sequence domains: SWIB/MDM2 domain
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 Chain: Q
Molecule details ›
Chain: Q
Length: 411 amino acids
Theoretical weight: 46.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q969G3 (Residues: 1-411; Coverage: 100%)
Gene names: BAF57, SMARCE1
Sequence domains: HMG (high mobility group) box
Zinc finger protein ubi-d4 Chain: R
Molecule details ›
Chain: R
Length: 391 amino acids
Theoretical weight: 44.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92785 (Residues: 1-391; Coverage: 100%)
Gene names: BAF45D, DPF2, REQ, UBID4
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-0968
Expression system: Homo sapiens

Quick links

Citations

41 review citations

Principles of nucleosome recognition by chromatin factors and enzymes.
McGinty et al. (2021)
40 more

4 mentions without citation

Chromatin remodelling comes into focus.
Sundaramoorthy et al. (2020)
3 more