› 6ltw

X-ray diffraction
1.65Å resolution

Crystal structure of Apo form of I122A/I330A variant of S-adenosylmethionine synthetase from Cryptosporidium hominis

Released:
DOI: 10.2210/pdb6ltw/pdb
PDB entry 6ltw coloured by chain, front view.
PDB entry 6ltw coloured by chain, side view.
PDB entry 6ltw coloured by chain, top view.
Source organism: Cryptosporidium hominis
Primary publication:
Engineered SAM Synthetases for Enzymatic Generation of AdoMet Analogs with Photocaging Groups and Reversible DNA Modification in Cascade Reactions.
Michailidou F, Klöcker N, Cornelissen NV, Singh RK, Peters A, Ovcharenko A, Kümmel D, Rentmeister A
OpenAccess logo Angew Chem Int Ed Engl 60 480-485 (2021)
PMID: 33017502

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 414 amino acids
Theoretical weight: 45.87 KDa
Source organism: Cryptosporidium hominis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0S4TKQ5 (Residues: 1-406; Coverage: 100%)
Gene name: CHUDEA7_2650
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Spacegroup: P21
Unit cell:
a: 62.877Å b: 100.36Å c: 66.842Å
α: 90° β: 95.86° γ: 90°
R-values:
R R work R free
0.158 0.156 0.191
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Enzyme-mediated bioorthogonal technologies: catalysts, chemoselective reactions and recent methyltransferase applications.
Jalali et al. (2021)
2 more

1 mention without citation

Engineered SAM Synthetases for Enzymatic Generation of AdoMet Analogs with Photocaging Groups and Reversible DNA Modification in Cascade Reactions.
Michailidou et al. (2021)