› 6lu2

X-ray diffraction
1.75Å resolution

Crystal structure of a substrate binding protein from Microbacterium hydrocarbonoxydans

Released:
DOI: 10.2210/pdb6lu2/pdb
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PDB entry 6lu2 coloured by chain, front view.
PDB entry 6lu2 coloured by chain, side view.
PDB entry 6lu2 coloured by chain, top view.
Source organism: Microbacterium hydrocarbonoxydans
Primary publication:
Structural basis of substrate recognition by the substrate binding protein (SBP) of a hydrazide transporter, obtained from Microbacterium hydrocarbonoxydans.
Shimamura K, Akiyama T, Yokoyama K, Takenoya M, Ito S, Sasaki Y, Yajima S
Biochem Biophys Res Commun 525 720-725 (2020)
PMID: 32143826

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-288711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Solute-binding protein family 5 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 511 amino acids
Theoretical weight: 53.87 KDa
Source organism: Microbacterium hydrocarbonoxydans
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A6I8WFP3 (Residues: 1-491; Coverage: 100%)
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 49.263Å b: 78.443Å c: 123.368Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.194 0.206
Expression system: Escherichia coli BL21

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