› 6lyu

Electron Microscopy
4.2Å resolution

Structure of the BAM complex

Released:
DOI: 10.2210/pdb6lyu/pdb
PDB entry 6lyu coloured by chain, front view.
PDB entry 6lyu coloured by chain, side view.
PDB entry 6lyu coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structures of the β-barrel assembly machine recognizing outer membrane protein substrates.
Xiao L, Han L, Li B, Zhang M, Zhou H, Luo Q, Zhang X, Huang Y
FASEB J 35 e21207 (2021)
PMID: 33368572
Related structures: EMD-30018

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-141767 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Outer membrane protein assembly factor BamA Chain: A
Molecule details ›
Chain: A
Length: 810 amino acids
Theoretical weight: 90.64 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A940 (Residues: 1-810; Coverage: 100%)
Gene names: JW0172, b0177, bamA, yaeT, yzzN, yzzY
Sequence domains:
Outer membrane protein assembly factor BamB Chain: B
Molecule details ›
Chain: B
Length: 400 amino acids
Theoretical weight: 42.96 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P77774 (Residues: 1-392; Coverage: 100%)
Gene names: JW2496, b2512, bamB, yfgL
Sequence domains:
Outer membrane protein assembly factor BamC Chain: C
Molecule details ›
Chain: C
Length: 344 amino acids
Theoretical weight: 36.88 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A903 (Residues: 1-344; Coverage: 100%)
Gene names: JW2462, b2477, bamC, dapX, nlpB
Sequence domains: NlpB/DapX lipoprotein
Outer membrane protein assembly factor BamD Chain: D
Molecule details ›
Chain: D
Length: 245 amino acids
Theoretical weight: 27.86 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0AC02 (Residues: 1-245; Coverage: 100%)
Gene names: JW2577, b2595, bamD, yfiO
Sequence domains: Outer membrane lipoprotein
Outer membrane protein assembly factor BamE Chain: E
Molecule details ›
Chain: E
Length: 119 amino acids
Theoretical weight: 13.14 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A937 (Residues: 1-113; Coverage: 100%)
Gene names: JW2598, b2617, bamE, smpA
Sequence domains: SmpA / OmlA family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-30018
Expression system: Escherichia coli K-12

Quick links

Citations

3 review citations

Targeting BAM for Novel Therapeutics against Pathogenic Gram-Negative Bacteria.
Overly Cottom et al. (2023)
2 more

1 mention without citation

Building Better Barrels - β-barrel Biogenesis and Insertion in Bacteria and Mitochondria.
Diederichs et al. (2021)