› 6lzh

X-ray diffraction
1.9Å resolution

Crystal structure of Alpha/beta hydrolase GrgF from Penicillium sp. sh18

Released:
DOI: 10.2210/pdb6lzh/pdb
PDB entry 6lzh coloured by chain, front view.
PDB entry 6lzh coloured by chain, side view.
PDB entry 6lzh coloured by chain, top view.
Source organism: Penicillium sp. sh18
Primary publication:
Molecular Basis for the Biosynthesis of an Unusual Chain-Fused Polyketide, Gregatin A.
Wang WG, Wang H, Du LQ, Li M, Chen L, Yu J, Cheng GG, Zhan MT, Hu QF, Zhang L, Yao M, Matsuda Y
J Am Chem Soc 142 8464-8472 (2020)
PMID: 32275405

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GrgF Chains: A, B
Molecule details ›
Chains: A, B
Length: 294 amino acids
Theoretical weight: 32.86 KDa
Source organism: Penicillium sp. sh18
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-1A
Spacegroup: P1
Unit cell:
a: 42.683Å b: 55.01Å c: 67.272Å
α: 101.708° β: 100.514° γ: 114.279°
R-values:
R R work R free
0.18 0.178 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Biosynthesis of Fungal Natural Products Involving Two Separate Pathway Crosstalk.
Dai et al. (2022)
2 more