› 6m0z

X-ray diffraction
2.88Å resolution

X-ray structure of Drosophila dopamine transporter with NET-like mutations (D121G/S426M/F471L) in L-norepinephrine bound form

Released:
DOI: 10.2210/pdb6m0z/pdb
PDB entry 6m0z coloured by chain, front view.
PDB entry 6m0z coloured by chain, side view.
PDB entry 6m0z coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of norepinephrine recognition and transport inhibition in neurotransmitter transporters.
Pidathala S, Mallela AK, Joseph D, Penmatsa A
OpenAccess logo Nat Commun 12 2199 (2021)
PMID: 33850134

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212475 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Sodium-dependent dopamine transporter Chain: A
Molecule details ›
Chain: A
Length: 536 amino acids
Theoretical weight: 60.1 KDa
Source organism: Drosophila melanogaster
Expression system: Homo sapiens
UniProt:
  • Canonical: Q7K4Y6 (Residues: 25-601; Coverage: 85%)
Gene names: CG8380, DAT, fmn
Sequence domains: Sodium:neurotransmitter symporter family
Antibody fragment (Fab) 9D5 Light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.31 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
Antibody fragment (Fab) 9D5 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 219 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda

Ligands and Environments

6 bound ligands:
Ligand CLR 1 x CLR
Ligand Y01 1 x Y01
Ligand DMU 1 x DMU
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand LNR 1 x LNR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 97.292Å b: 142.801Å c: 168.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.243
Expression systems:
  • Homo sapiens
  • Spodoptera frugiperda

Quick links

Citations

2 review citations

Molecular mechanisms of action of stimulant novel psychoactive substances that target the high-affinity transporter for dopamine.
Sahai et al. (2021)
1 more

2 mentions without citation

Anti-Entry Activity of Natural Flavonoids against SARS-CoV-2 by Targeting Spike RBD.
Meng et al. (2023)
1 more