› 6m2i

X-ray diffraction
2.45Å resolution

Structure of the 2-Aminoisobutyric acid Monooxygenase Hydroxylase

Released:
DOI: 10.2210/pdb6m2i/pdb
PDB entry 6m2i coloured by chain, front view.
PDB entry 6m2i coloured by chain, side view.
PDB entry 6m2i coloured by chain, top view.
Source organism: Rhodococcus wratislaviensis
Primary publication:
A three-component monooxygenase from Rhodococcus wratislaviensis may expand industrial applications of bacterial enzymes.
Hibi M, Fukuda D, Kenchu C, Nojiri M, Hara R, Takeuchi M, Aburaya S, Aoki W, Mizutani K, Yasohara Y, Ueda M, Mikami B, Takahashi S, Ogawa J
OpenAccess logo Commun Biol 4 16 (2021)
PMID: 33398074

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105013 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Amidohydrolase-related domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 385 amino acids
Theoretical weight: 43.47 KDa
Source organism: Rhodococcus wratislaviensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A402C2V4 (Residues: 1-373; Coverage: 100%)
Gene name: Rhow_000804
Sequence domains: Amidohydrolase
Amidohydrolase-related domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 378 amino acids
Theoretical weight: 42.6 KDa
Source organism: Rhodococcus wratislaviensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A402C2Q3 (Residues: 1-378; Coverage: 100%)
Gene name: Rhow_000803
Sequence domains: Amidohydrolase

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 5 x SO4
Ligand EDO 17 x EDO
Ligand FE 2 x FE
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2221
Unit cell:
a: 123.751Å b: 206.684Å c: 91.489Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.236
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Rhodococcus strains as a good biotool for neutralizing pharmaceutical pollutants and obtaining therapeutically valuable products: Through the past into the future.
Ivshina et al. (2022)
1 other article

1 mention without citation

Enzymatic Hydroxylation of Aliphatic C-H Bonds by a Mn/Fe Cofactor.
Powell et al. (2023)