6m9w

X-ray diffraction
1.5Å resolution

Structure of Mg-free KRAS4b (2-169) bound to GDP with the switch-I in fully open conformation

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 170 amino acids
Theoretical weight: 19.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01116 (Residues: 2-169; Coverage: 89%)
  • Best match: P01116-2 (Residues: 2-169)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P321
Unit cell:
a: 77.858Å b: 77.858Å c: 55.726Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.145 0.142 0.181
Expression system: Escherichia coli BL21(DE3)