› 6mav

X-ray diffraction
2.37Å resolution

Complex of tissue inhibitor of metalloproteinase-1 (TIMP-1) mutant L34G with matrix metalloproteinase-3 catalytic domain (MMP-3cd)

Released:
DOI: 10.2210/pdb6mav/pdb
PDB entry 6mav coloured by chain, front view.
PDB entry 6mav coloured by chain, side view.
PDB entry 6mav coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Directed evolution of the metalloproteinase inhibitor TIMP-1 reveals that its N- and C-terminal domains cooperate in matrix metalloproteinase recognition.
Raeeszadeh-Sarmazdeh M, Greene KA, Sankaran B, Downey GP, Radisky DC, Radisky ES
J Biol Chem 294 9476-9488 (2019)
PMID: 31040180

Function and Biology Details

Reaction catalysed: 
Preferential cleavage where P1', P2' and P3' are hydrophobic residues.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133885 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Stromelysin-1 Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 18.89 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08254 (Residues: 100-267; Coverage: 37%)
Gene names: MMP3, STMY1
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)
Metalloproteinase inhibitor 1 Chain: B
Molecule details ›
Chain: B
Length: 184 amino acids
Theoretical weight: 20.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01033 (Residues: 24-207; Coverage: 100%)
Gene names: CLGI, TIMP, TIMP1
Sequence domains: Tissue inhibitor of metalloproteinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P6522
Unit cell:
a: 69.703Å b: 69.703Å c: 321.326Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.217 0.298
Expression system: Homo sapiens

Quick links

Citations

7 review citations

The Rebirth of Matrix Metalloproteinase Inhibitors: Moving Beyond the Dogma.
Fields GB. (2019)
6 more

2 mentions without citation

Directed evolution of the metalloproteinase inhibitor TIMP-1 reveals that its N- and C-terminal domains cooperate in matrix metalloproteinase recognition.
Raeeszadeh-Sarmazdeh et al. (2019)
1 more