› 6mb3

Electron Microscopy
3.37Å resolution

Cryo-EM structure of the circumsporozoite protein of Plasmodium falciparum with a vaccine-elicited antibody reveals maturation of inter-antibody contacts

Released:
DOI: 10.2210/pdb6mb3/pdb
PDB entry 6mb3 coloured by chain, front view.
PDB entry 6mb3 coloured by chain, side view.
PDB entry 6mb3 coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of P. falciparum circumsporozoite protein with a vaccine-elicited antibody is stabilized by somatically mutated inter-Fab contacts.
Oyen D, Torres JL, Cottrell CA, Richter King C, Wilson IA, Ward AB
OpenAccess logo Sci Adv 4 eaau8529 (2018)
PMID: 30324137
Related structures: EMD-9065

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonadecamer (preferred)
PDBe Complex ID:
PDB-CPX-209618 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Circumsporozoite protein Chain: E
Molecule details ›
Chain: E
Length: 278 amino acids
Theoretical weight: 30.23 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7K740 (Residues: 26-383; Coverage: 73%)
Gene names: CSP, PF3D7_0304600
Sequence domains: Thrombospondin type 1 domain
Immunoglobulin gamma-1 heavy chain Chains: A, B, C, D, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, F, G, H, I, J
Length: 225 amino acids
Theoretical weight: 24.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P0DOX5 (Residues: 1-99, 100-223; Coverage: 50%)
Sequence domains:
Fab311 light chain Chains: K, L, M, N, O, P, Q, R, S
Molecule details ›
Chains: K, L, M, N, O, P, Q, R, S
Length: 218 amino acids
Theoretical weight: 22.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.37Å
Relevant EMDB volumes: EMD-9065
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

9 review citations

Malaria vaccines since 2000: progress, priorities, products.
Duffy et al. (2020)
8 more

2 mentions without citation

Structural and biophysical correlation of anti-NANP antibodies with in vivo protection against P. falciparum.
Pholcharee et al. (2021)
1 more