› 6mbe

X-ray diffraction
2.25Å resolution

Human Mcl-1 in complex with the designed peptide dM7

Released:
DOI: 10.2210/pdb6mbe/pdb
Model geometry
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PDB entry 6mbe coloured by chain, front view.
PDB entry 6mbe coloured by chain, side view.
PDB entry 6mbe coloured by chain, top view.
Source organisms:
Primary publication:
Tertiary Structural Motif Sequence Statistics Enable Facile Prediction and Design of Peptides that Bind Anti-apoptotic Bfl-1 and Mcl-1.
Frappier V, Jenson JM, Zhou J, Grigoryan G, Keating AE
Structure 27 606-617.e5 (2019)
PMID: 30773399

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167061 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Induced myeloid leukemia cell differentiation protein Mcl-1 Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07820 (Residues: 172-323; Coverage: 43%)
Gene names: BCL2L3, MCL1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
dM7 Chain: B
Molecule details ›
Chain: B
Length: 24 amino acids
Theoretical weight: 2.61 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: OTHER
Spacegroup: P3221
Unit cell:
a: 80.758Å b: 80.758Å c: 57.95Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.221
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

5 review citations

Recent advances in de novo protein design: Principles, methods, and applications.
Pan et al. (2021)
4 more

2 mentions without citation

Tertiary Structural Motif Sequence Statistics Enable Facile Prediction and Design of Peptides that Bind Anti-apoptotic Bfl-1 and Mcl-1.
Frappier et al. (2019)
1 more

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