› 6mbf

X-ray diffraction
1.54Å resolution

GphF Dehydratase 1

Released:
DOI: 10.2210/pdb6mbf/pdb
PDB entry 6mbf coloured by chain, front view.
PDB entry 6mbf coloured by chain, side view.
PDB entry 6mbf coloured by chain, top view.
Source organism: Archangium violaceum
Primary publication:
Molecular Basis for Olefin Rearrangement in the Gephyronic Acid Polyketide Synthase.
Dodge GJ, Ronnow D, Taylor RE, Smith JL
ACS Chem Biol 13 2699-2707 (2018)
PMID: 30179448

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyketide synthase Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 32.7 KDa
Source organism: Archangium violaceum
Expression system: Escherichia coli
UniProt:
  • Canonical: U6BSB2 (Residues: 1697-1984; Coverage: 6%)
Gene name: gphF
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 41.9Å b: 74.418Å c: 86.836Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.222
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases.
Barajas et al. (2019)
1 more

2 mentions without citation

An Unconventional Melanin Biosynthesis Pathway in Ustilago maydis.
Reyes-Fernández et al. (2021)
1 more