› 6mgh

X-ray diffraction
1.95Å resolution

X-ray structure of monomeric near-infrared fluorescent protein miRFP670nano

Released:
DOI: 10.2210/pdb6mgh/pdb
PDB entry 6mgh coloured by chain, front view.
PDB entry 6mgh coloured by chain, side view.
PDB entry 6mgh coloured by chain, top view.
Source organism: Acaryochloris marina
Primary publication:
Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing.
Oliinyk OS, Shemetov AA, Pletnev S, Shcherbakova DM, Verkhusha VV
OpenAccess logo Nat Commun 10 279 (2019)
PMID: 30655515

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-167065 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
miRFP670nano Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 163 amino acids
Theoretical weight: 18.82 KDa
Source organism: Acaryochloris marina
Expression system: Escherichia coli
Structure domains: Beta-Lactamase

Ligands and Environments

3 bound ligands:
Ligand JRA 8 x JRA
Ligand GOL 4 x GOL
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 187.598Å b: 72.124Å c: 136.698Å
α: 90° β: 129.99° γ: 90°
R-values:
R R work R free
0.183 0.182 0.239
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Advanced imaging and labelling methods to decipher brain cell organization and function.
Choquet et al. (2021)
19 more

6 mentions without citation

Deep-tissue SWIR imaging using rationally designed small red-shifted near-infrared fluorescent protein.
Oliinyk et al. (2023)
5 more