› 6mhf

X-ray diffraction
2Å resolution

Galphai3 co-crystallized with GIV/Girdin

Released:
DOI: 10.2210/pdb6mhf/pdb
PDB entry 6mhf coloured by chain, front view.
PDB entry 6mhf coloured by chain, side view.
PDB entry 6mhf coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for GPCR-independent activation of heterotrimeric Gi proteins.
Kalogriopoulos NA, Rees SD, Ngo T, Kopcho NJ, Ilatovskiy AV, Sun N, Komives EA, Chang G, Ghosh P, Kufareva I
Proc Natl Acad Sci U S A 116 16394-16403 (2019)
PMID: 31363053

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140362 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-3 Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.07 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P08753 (Residues: 26-354; Coverage: 93%)
Gene names: Gnai-3, Gnai3
Sequence domains: G-protein alpha subunit
Girdin Chain: C
Molecule details ›
Chain: C
Length: 31 amino acids
Theoretical weight: 3.31 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q3V6T2 (Residues: 1672-1702; Coverage: 2%)
Gene names: APE, CCDC88A, GRDN, KIAA1212

Ligands and Environments

2 bound ligands:
Ligand GDP 1 x GDP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P43212
Unit cell:
a: 83.78Å b: 83.78Å c: 141.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.239
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Do All Roads Lead to Rome in G-Protein Activation?
Ghosh et al. (2020)
2 more

5 mentions without citation

Structural basis for GPCR-independent activation of heterotrimeric Gi proteins.
Kalogriopoulos et al. (2019)
4 more