› 6mit

X-ray diffraction
3.2Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-493928 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Lipopolysaccharide export system ATP-binding protein LptB Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 241 amino acids
Theoretical weight: 26.82 KDa
Source organism: Enterobacter cloacae subsp. cloacae ATCC 13047
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3CR83 (Residues: 1-241; Coverage: 100%)
Gene name: ECL_04583
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Lipopolysaccharide export system protein LptC Chains: C, H
Molecule details ›
Chains: C, H
Length: 195 amino acids
Theoretical weight: 22.26 KDa
Source organism: Enterobacter cloacae subsp. cloacae ATCC 13047
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3CU18 (Residues: 1-191; Coverage: 100%)
Gene names: ECL_04581, lptC
Sequence domains: Lipopolysaccharide-assembly, LptC-related
Lipopolysaccharide export system permease protein LptF Chains: F, I
Molecule details ›
Chains: F, I
Length: 366 amino acids
Theoretical weight: 40.58 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A421IFY4 (Residues: 1-366; Coverage: 100%)
Gene names: N5E88_04445, N7383_01945, RRL09_02130, lptF
Sequence domains: Lipopolysaccharide export system permease LptF/LptG
Lipopolysaccharide export system permease protein LptG Chains: G, J
Molecule details ›
Chains: G, J
Length: 360 amino acids
Theoretical weight: 39.57 KDa
Source organism: Enterobacter cloacae subsp. cloacae ATCC 13047
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3CQA2 (Residues: 1-360; Coverage: 100%)
Gene name: ECL_04592
Sequence domains: Lipopolysaccharide export system permease LptF/LptG

Ligands and Environments

3 bound ligands:
Ligand SO4 11 x SO4
Ligand CL 1 x CL
Ligand NOV 1 x NOV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Unit cell:
a: 116.427Å b: 156.984Å c: 295.518Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.28 0.279 0.32
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Pushing the envelope: LPS modifications and their consequences.
Simpson et al. (2019)
16 more

11 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
10 more