› 6mlt

X-ray diffraction
1.9Å resolution

Crystal structure of the V. cholerae biofilm matrix protein Bap1

Released:
DOI: 10.2210/pdb6mlt/pdb
PDB entry 6mlt coloured by chain, front view.
PDB entry 6mlt coloured by chain, side view.
PDB entry 6mlt coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Primary publication:
The 1.9 Å crystal structure of the extracellular matrix protein Bap1 from Vibrio cholerae provides insights into bacterial biofilm adhesion.
Kaus K, Biester A, Chupp E, Lu J, Visudharomn C, Olson R
J Biol Chem 294 14499-14511 (2019)
PMID: 31439670

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemolysin beta-prism lectin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 613 amino acids
Theoretical weight: 66.24 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KQW0 (Residues: 27-691; Coverage: 91%)
Gene name: VC_1888
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand NA 5 x NA
Ligand FLC 1 x FLC
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P41212
Unit cell:
a: 70.951Å b: 70.951Å c: 303.957Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.176
Expression system: Escherichia coli BL21

Quick links

Citations

3 citation in other articles

Outer Membrane Vesicles Released From Aeromonas Strains Are Involved in the Biofilm Formation.
Seike et al. (2020)
2 more

2 mentions without citation

Searching for the Secret of Stickiness: How Biofilms Adhere to Surfaces.
Jiang et al. (2021)
1 more