› 6mpv

Electron Microscopy
7.17Å resolution

Cryo-electron microscopy structure of Plasmodium falciparum Rh5/CyRPA/Ripr invasion complex

Released:
DOI: 10.2210/pdb6mpv/pdb
PDB entry 6mpv coloured by chain, front view.
PDB entry 6mpv coloured by chain, side view.
PDB entry 6mpv coloured by chain, top view.
Source organisms:
Primary publication:
Structure of Plasmodium falciparum Rh5-CyRPA-Ripr invasion complex.
Wong W, Huang R, Menant S, Hong C, Sandow JJ, Birkinshaw RW, Healer J, Hodder AN, Kanjee U, Tonkin CJ, Heckmann D, Soroka V, Søgaard TMM, Jørgensen T, Duraisingh MT, Czabotar PE, de Jongh WA, Tham WH, Webb AI, Yu Z, Cowman AF
Nature 565 118-121 (2019)
PMID: 30542156
Related structures: EMD-9192

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-109680 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cysteine-rich protective antigen Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 39.27 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: Q8IFM8 (Residues: 31-362; Coverage: 99%)
Gene names: CyRPA, PF3D7_0423800, pfd1130w
Sequence domains: Cysteine-Rich Protective Antigen 6 bladed domain
Rh5 coiled-coil domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 330 amino acids
Theoretical weight: 39.8 KDa
Source organism: Plasmodium falciparum
Expression system: Insect cell expression vector pTIE1
UniProt:
  • Canonical: B2L3N7 (Residues: 175-504; Coverage: 63%)
Sequence domains: Rh5 coiled-coil domain
PfRipr Chain: C
Molecule details ›
Chain: C
Length: 16 amino acids
Theoretical weight: 1.38 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Drosophila nebulosa
PfRipr Chain: D
Molecule details ›
Chain: D
Length: 5 amino acids
Theoretical weight: 444 Da
Source organism: Plasmodium falciparum 3D7
Expression system: Drosophila nebulosa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.17Å
Relevant EMDB volumes: EMD-9192
Expression systems:
  • Insect cell expression vector pTIE1
  • Drosophila nebulosa

Quick links

Citations

7 review citations

Antibodies against Plasmodium falciparum malaria at the molecular level.
Julien et al. (2019)
6 more

9 mentions without citation

The RH5-CyRPA-Ripr Complex as a Malaria Vaccine Target.
Ragotte et al. (2020)
8 more